Hieff™ Magnetic Blood DNA Kit _ 18504ES

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The Hieff™ Magnetic Blood DNA Kit provides fast and easy methods for purification of total DNA from blood samples. This product is based on the purification method of high binding magnetic particles. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The genomic DNA is suitable for various experiments, such as enzyme digestion, PCR,qPCR, etc. Features High yield and purity: OD260/280 ratio between 1.8–2.0. Broad sample compatibility: Works with fresh blood, frozen blood, blood cells, and more. Compatible with instruments: Suitable for high-throughput extraction on various automatic nucleic acid extractors. Safe and non-toxic: Free of harmful substances like phenol and chloroform. Components Part Components No. Name 18504ES50（50 rxn） 18504ES70（200 rxn） Part I 18504-A Proteinase K Solution（20 mg/mL） 1 mL 1 mL×4 Part II 18504-B Magnetic beads Suspension 1 mL 1 mL×4 18504-C Lysis buffer 15 mL 60 mL 18504-D Rinse buffer* 52 mL 70 mL×3 18504-E Wash buffer* 16 mL 22 mL×3 18504-F Elution buffer 5 mL 20 mL Application Blood gDNA extraction from blood, plasma, serum, or lymph samples. Figures Extraction workflow Whole Blood RNA Extraction kit 18504 Supplier M* Sample Nucleic Acids (ng/μL) 260/280 260/230 Nucleic Acids (ng/μL) 260/280 260/230 Sample 1 68.4 1.858 1.778 62.4 1.691 1.706 Sample 2 78.2 1.708 1.997 76.4 1.702 1.832 Sample 3 116 1.798 1.806 104 1.687 1.615 Sample 4 112 1.896 1.888 92.4 1.680 1.791 Sample 5 124.8 1.737 1.703 116.4 1.708 1.510 Sample 6 97.2 1.658 1.877 88.2 1.707 1.621 Mean Value 99.43 1.78 1.84 89.97 1.70 1.68 Figure 1. Hieff™ Magnetic Blood DNA Kit extracts more blood DNA than kits from other suppliers. 300 μL of fresh blood in EDTA storage tubes was extracted simultaneously using the kits of 18504 and supplier M*, with a 96-channel nucleic acid extractor and 70 μL elution. The results showed that the extraction rate of blood gDNA by 18504 was higher than that of supplier M*. Supports extraction from swab samples kit 18504 Supplier K* Swab sample Concentration (ng/μL) 260/280 260/230 Ct Mean Concentration (ng/μL) 260/280 260/230 Ct Mean Sample 1 3.015 1.901 1.227 9.901 4.907 0.998 0.011 10.778 3.102 1.807 1.157 9.344 14.454 0.516 0.028 8.641 3.542 1.724 1.187 9.547 5.652 0.688 0.016 7.514 Sample 2 5.333 1.982 1.794 8.539 6.041 1.571 0.009 7.788 7.466 1.803 1.702 7.823 6.765 1.022 0.021 9.414 7.139 1.975 1.721 6.777 7.203 0.645 0.024 10.893 Mean Value 4.932 1.871 1.462 8.663 7.50 2 0.913 0.022 9.171 Figure 2. Performance of Hieff™ Magnetic Blood DNA Kit from swab sample Test reagent: 18504ES and supplier K* ; elution volume: 70 μL. Test scheme: AP-96 fully automated nucleic acid extractor; nanodrop detects DNA concentration and purity. 10 μL of each eluate was analyzed by qPCR using the HEK293 Residual DNA Detection Kit (Cat#41302). Test results: 18504 showed brighter bands, higher purity, and lower average Ct values compared to the competitor Show more