The Hieff™ Fungal DNA Kit is suitable for extracting genomic DNA from 100 mg of fresh or 20 mg of dried fungal tissue within 30 minutes. The silica matrix material used in the centrifuge adsorption column is a proprietary new material from our company, which, combined with our unique buffer formulation, can maximize the removal of cellular metabolites, proteins, and other impurities. The extracted DNA has high purity and stable, reliable quality, making it suitable for various downstream applications such as restriction digestion, PCR, library construction, and more. Features 1. Typical A260/A280 & A260/A230 ≥ 1.8. 2. Capable of recovering genomic DNA sized fragments from up to and above 40 kb. 3. 100 mg of fresh or 20 mg of dried fungal tissue within 30 minutes. Applications All sensitive downstream applications such as qPCR and Next-Generation Sequencing. Specifications Cat.No. 18812ES50 / 18812ES70 Size 50 T / 200 T Components Category Components No. Name 18812ES50 18812ES70 Part I 18812-A RNase A (10 mg/mL) 250 µL 1 mL Part II 18812-B Hieff DNA Column F1 50 pieces 200 pieces 18812-C 2 mL Collection Tube F1 50 pieces 200 pieces 18812-D Lysis buffer LB 25 mL 100 mL 18812-E Binding buffer BD 10 mL 40 mL 18812-F Protein removal buffer PL* 15 mL 50 mL 18812-G Wash Buffer* 13 mL 50 mL 18812-H Elution buffer 10 mL 20 mL Shipping and Storage Part I components should be stored at -25~-15℃, valid for 2 years. Part II components should be stored at room temperature, valid for 2 years. Figures Documents: Safety Data Sheet 18812_MSDS_HB230223 Manuals 18812_Manual_Ver.EN20250825 Citations & References: 1. Zhang J, Tian R, Liu J, Yuan J, Zhang S, Chi Z, Yu W, Yu Q, Wang Z, Chen S, Li M, Yang D, Hu T, Deng Q, Lu X, Yang Y, Zhou R, Zhang X, Liu W, Wang D. A two-front nutrient supply environment fuels small intestinal physiology through differential regulation of nutrient absorption and host defense. Cell. 2024 Oct 31;187(22):6251-6271.e20. doi: 10.1016/j.cell.2024.08.012. Epub 2024 Oct 19. PMID: 39427662.(IF:45)
