The Hieff™ Cell/Tissue miRNA Kit is suitable for the extraction of total RNA, miRNA, and various small RNAs from all samples that can be extracted using the TRIzol method, including various animal and plant tissues, bacterial tissues, and cultured cells. The silica-based material used in the centrifuge adsorption column is a proprietary new material from our company, which, combined with our unique lysis buffer formulation, can maximize the recovery of high-purity RNA and miRNA. The extracted RNA has high purity, stable and reliable quality, is suitable for RT-qPCR and other downstream applications. Features • Efficient isolation of small RNA-containing total RNA • Enrich for RNA larger than 10 nt to increase sensitivity in downstream analyses • Simple 30 minute procedure • Ideal for miRNA, siRNA, shRNA, and snRNA analysis • Compatible with virtually all cell and tissue types，samples consisting of 5×10 6 ~ 1×10 7 cultured cells or 50–100 mg tissue. Applications RT-qPCR, microarray analysis, Northern blotting, NGS Specifications Cat.No. 19331ES08 / 19331ES50 Size 5 T / 50 T Components Category Components No. Name 19331ES08 19331ES50 Part I 19331-A LB Buffer M1 5 mL 50 mL Part II 19331-B Hieff™ RNA Column M1 5 pieces 50 pieces 19331-C miRNA Column M1 5 pieces 50 pieces 19331-D 2 mL Collection Tube M1 10 pieces 100 pieces 19331-E PL Buffer M1* 1.2 mL 12 mL 19331-F Wash Buffer M1* 1.3 mL 13 mL 19331-G RNase-free H2O 1 mL 5 mL Shipping and Storage Part I components should be stored at 4~8℃ for 2 years. Part II components should be stored at room temperature for 2 years. Figures Documents: Safety Data Sheet 19331_MSDS_HB250626 Manuals 19331_Manual_Ver.EN20250825 Citations & References: [1] Dong Z, Zheng N, Hu C, et al. Nosema bombycis microRNA-like RNA 8 (Nb-milR8) Increases Fungal Pathogenicity by Modulating BmPEX16 Gene Expression in Its Host, Bombyx mori. Microbiol Spectr. 2021;9(2):e0104821. doi:10.1128/Spectrum.01048-21(IF:7.171)[2] Hu C, Dong Z, Deng B, et al. MicroRNA-6498-5p Inhibits Nosema bombycis Proliferation by Downregulating BmPLPP2 in Bombyx mori. J Fungi (Basel). 2021;7(12):1051. Published 2021 Dec 8. doi:10.3390/jof7121051(IF:5.816)[3] Teng JW, Bian SS, Kong P, Chen YG. Icariin triggers osteogenic differentiation of bone marrow stem cells by up-regulating miR-335-5p. Exp Cell Res. 2022;414(2):113085. doi:10.1016/j.yexcr.2022.113085(IF:3.905)[4] Cui Z, Li Y, Liu G, Jiang Y. miR-103a-3p Silencing Ameliorates Calcium Oxalate Deposition in Rat Kidney by Activating the UMOD/TRPV5 Axis. Dis Markers. 2022;2022:2602717. Published 2022 Feb 23. doi:10.1155/2022/2602717(IF:3.434)[5] Yi L, Liu J, Deng M, Zuo H, Li M. Emodin inhibits viability, proliferation and promotes apoptosis of hypoxic human pulmonary artery smooth muscle cells via targeting miR-244-5p/DEGS1 axis. BMC Pulm Med. 2021;21(1):252. Published 2021 Jul 31. doi:10.1186/s12890-021-01616-1(IF:3.317)[6] Liu D, Wan L, Gong H, Chen S, Kong Y, Xiao B. Sevoflurane promotes the apoptosis of laryngeal squamous cell carcinoma in-vitro and inhibits its malignant progression via miR-26a/FOXO1 axis. Bioengineered. 2021;12(1):6364-6376. doi:10.1080/21655979.2021.1962684(IF:3.269)[7] Shi M, Chen X, Li H, Zheng L. δ-tocotrienol suppresses the migration and angiogenesis of trophoblasts in preeclampsia and promotes their apoptosis via miR-429/ ZEB1 axis. Bioengineered. 2021;12(1):1861-1873. doi:10.1080/21655979.2021.1923238(IF:3.269)[8] Bi X, Jiang Z, Luan Z, Qiu D. Crocin exerts anti-proliferative and apoptotic effects on cutaneous squamous cell carcinoma via miR-320a/ATG2B. Bioengineered. 2021;12(1):4569-4580. doi:10.1080/21655979.2021.1955175(IF:3.269)[9] Hu Y, Wu L, Jiang L, et al. Notoginsenoside R2 reduces Aβ25-35-induced neuronal apoptosis and inflammation via miR-27a/SOX8/β-catenin axis. Hum Exp Toxicol. 2021;40(12_suppl):S347-S358. doi:10.1177/09603271211041996(IF:2.903)[10] Li Y, Qin G, Du J, Yue P, Zhang Y, Hou N. circRNA LDLRAD3 Enhances the Malignant Behaviors of NSCLC Cells via the miR-20a-5p-SLC7A5 Axis Activating the mTORC1 Signaling Pathway. J Healthc Eng. 2022;2022:2373580. Published 2022 Jan 6. doi:10.1155/2022/2373580(IF:2.682)[11] Sun Y, Lu X, Li H, Li X. Dihydroartemisinin inhibits IL-6-induced epithelial-mesenchymal transition in laryngeal squamous cell carcinoma via the miR-130b-3p/STAT3/β-catenin signaling pathway. J Int Med Res. 2021;49(11):3000605211009494. doi:10.1177/03000605211009494(IF:1.671)[12] Guo X, Ji Q, Wu M, Ma W. Naringin attenuates acute myocardial ischemia-reperfusion injury via miR- 126/GSK-3β/β-catenin signaling pathway. Acta Cir Bras. 2022;37(1):e370102. Published 2022 Apr 8. doi:10.1590/acb370102(IF:1.388) [13] Sun B, Huang Y, Castro LFC, Yang S, Huang S, Jin W, Zhou H, Ijiri S, Luo Y, Gao J, Cao X. The chromosome-level genome and key genes associated with mud-dwelling behavior and adaptations of hypoxia and noxious environments in loach (Misgurnus anguillicaudatus). BMC Biol. 2023 Feb 1;21(1):18. doi: 10.1186/s12915-023-01517-1. (IF:7.3)
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