After cell lysis, the protein crude extract contains a large amount of endogenous proteases that can degrade proteins in the cell extract. To prevent the degradation of the target protein by proteases during protein purification, protease inhibitors are required to inhibit their activity. This product is a mixture of various common protease inhibitors, including AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, Pepstatin A, and other components, which broadly inhibit a variety of proteases from plants, animals, bacteria, yeast, and other tissues or cells, such as serine proteases, cysteine proteases, aminopeptidases, and acidic proteases. Additionally, this product does not contain EDTA (a metalloproteinase inhibitor), making it particularly suitable for proteins whose active centers depend on metal ions. It is compatible with subsequent immobilized metal-chelate affinity chromatography (IMAC), 2-D gel electrophoresis, and native gel electrophoresis for protein analysis experiments. This product is provided as a 100× stock solution dissolved in DMSO and can be used for WB, Co-IP, Pull-down assay, IF, IHC, kinase research, and other analyses. Components Components No. Name 20124ES03 20124ES10 20124ES60 20124 InStab ™ Protease Inhibitor Cocktail,EDTA-free,100×DMSO Stock Solution 1 mL 10×1 mL 100×1 mL Shipping and Storage The product is shipped with ice pack and can be stored at -15~-25℃ for 1 year. Instructions Before use, allow the product to warm to room temperature. Add to the sample solution at a ratio of 1:100 (v/v) before sample analysis, and mix well. Notes 1. This product has broad-spectrum protease inhibitory activity but does not possess phosphatase inhibitory activity. For research on phosphorylated proteins, our phosphatase inhibitor mixture (100×) (Cat#20109) can be used. 2. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety！ 3. For research use only! Documents: Safety Data Sheet 20124-MSDS-HB250826.pdf Manuals 20124_Manual_CN20250102_EN Citations & References: [1] Xiang Y, Bian X, Wei T, et al. ZmMPK5 phosphorylates ZmNAC49 to enhance oxidative stress tolerance in maize. New Phytol. 2021;232(6):2400-2417. doi:10.1111/nph.17761(IF:10.152) [2] Liu J, Yang Y, Liu X, Widjaya AS, Jiang B, Jiang Y. Macrophage-biomimetic anti-inflammatory liposomes for homing and treating of aortic dissection. J Control Release. 2021;337:224-235. doi:10.1016/j.jconrel.2021.07.032(IF:9.776) [3] Liu L, Li Y, Cao D, et al. SIRT3 inhibits gallbladder cancer by induction of AKT-dependent ferroptosis and blockade of epithelial-mesenchymal transition. Cancer Lett. 2021;510:93-104. doi:10.1016/j.canlet.2021.04.007(IF:8.679) [4] Dou R, Qian J, Wu W, et al. Suppression of steroid 5α-reductase type I promotes cellular apoptosis and autophagy via PI3K/Akt/mTOR pathway in multiple myeloma. Cell Death Dis. 2021;12(2):206. Published 2021 Feb 24. doi:10.1038/s41419-021-03510-4(IF:8.469) [5] Hu W, Wang Z, Zhang H, et al. Chk1 Inhibition Ameliorates Alzheimer's Disease Pathogenesis and Cognitive Dysfunction Through CIP2A/PP2A Signaling. Neurotherapeutics. 2022;19(2):570-591. doi:10.1007/s13311-022-01204-z(IF:7.620) [6] Sun J, Guo Y, Fan Y, Wang Q, Zhang Q, Lai D. Decreased expression of IDH1 by chronic unpredictable stress suppresses proliferation and accelerates senescence of granulosa cells through ROS activated MAPK signaling pathways. Free Radic Biol Med. 2021;169:122-136. doi:10.1016/j.freeradbiomed.2021.04.016(IF:7.376) [7] Liu YJ, Xu WH, Fan LM, et al. Polydatin alleviates DSS- and TNBS-induced colitis by suppressing Th17 cell differentiation via directly inhibiting STAT3 [published online ahead of print, 2022 Jun 29]. Phytother Res. 2022;10.1002/ptr.7533IF: 6.1 Q1 . doi:10.1002/ptr.7533(IF:6.388) [8] Zhang X, Cai Z, Wu M, Huangfu X, Li J, Liu X. Adipose Stem Cell-Derived Exosomes Recover Impaired Matrix Metabolism of Torn Human Rotator Cuff Tendons by Maintaining Tissue Homeostasis. Am J Sports Med. 2021;49(4):899-908. doi:10.1177/0363546521992469(IF:6.203) [9] Gao C, Wang SW, Lu JC, et al. KSR2-14-3-3ζ complex serves as a biomarker and potential therapeutic target in sorafenib-resistant hepatocellular carcinoma. Biomark Res. 2022;10(1):25. Published 2022 Apr 25. doi:10.1186/s40364-022-00361-9(IF:6.148) [10] Xu C, Zhang M, Bian L, Li Y, Yao Y, Li D. N-glycosylated SGK196 suppresses the metastasis of basal-like breast cancer cells. Oncogenesis. 2020;9(1):4. Published 2020 Jan 8. doi:10.1038/s41389-019-0188-1(IF:6.119)
