This product consists of 12 highly purified and prestained proteins ranging in molecular weight from 10 kDa to 245 kDa, among them, 72 kDa is orange band, 25 kDa is green band, 10/15/20/35/ 45/60/100/140/180/245 kDa are blue bands, the protein concentration of each band was about 0.1-0.4 mg/mL. Using this product, the state of protein electrophoresis and the effect of membrane transfer can be dynamically observed. After SDS-PAGE electrophoresis, the color bands were transferred to PVDF membrane and NC membrane. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents! After the prestained protein is combined with the dye, the molecular weight will change in the gel of different concentrations. This product is only for reference when judging the molecular weight of the target protein. Components Components No. Name 20352ES72 20352ES76 20352ES90 20352 GoldBand™ 3-color High Range Protein Marker (10-245 kDa) 250 μL 2×250 μL 10×250 μL Storage The product should be stored at -25℃ ~ -15℃ for two years.For regular use, it can be placed at 4℃, valid for three months. It is recommended to store in aliquots to avoid repeated freezing and thawing! Stock solution composition 62.5 mM Tris-H3PO4(pH 7.5), 2 mM EDTA, 2% (W/V) SDS, 33% (W/V) Glycerol, 5 mM DTT, 0.02% (V/V) proclin300 Instructions 1. After the product is thawed at room temperature,mix gently to fully dissolve the precipitate. 2. Then take an appropriate amount of this product into the gel hole. mini-gel: 3-5 μL; when the thickness of the gel is greater than 1.5 mm or the macromolecular bands need to be deepened, the loading volume can be appropriately increased: 5-10 μL. Notes 1. The product needs to be returned to room temperature before use to fully dissolve the precipitate. Incomplete protein denaturation at low temperature may lead to different degrees of dispersion of electrophoresis bands. 2. In western blot experiments, large proteins (>100 kDa) in the product may require longer transfer times or higher transfer voltages to complete transfer. 3. This product contains SDS, and the protein has been denatured, so it should not be used as a molecular weight reference standard for natural protein molecular electrophoresis. 4. The product will have deviations in protein size under different electrophoresis conditions, but after they are calibrated by non-prestained protein standards in the same buffer system, they can be used for protein determination of similar molecular weights. 5. At low concentration of gel, low molecular weight protein will swim on the dye front. 6. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents! 7. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety！ 8. For research use only! Figure1. 4-20% SDS-PAGE electrophoresis results Attached table Under different electrophoresis buffer conditions, each band of this product indicates molecular weight Bands colour Tris-Glycine Bis-Tris Hepes-Tris 1 Blue 245 240 235 2 Blue 180 173 175 3 Blue 140 135 136 4 Blue 100 94 98 5 Red 72 65 66 6 Blue 60 57 58 7 Blue 45 43 44 8 Blue 35 32 33 9 Green 25 23 24 10 Blue 20 19 20 11 Blue 15 14 15 12 Blue 10 10 10 Customer Feedback 1.From Columbia University: Sharp bands and vivid colors. 2.From Boston Children's Hospital: Crisp bands, vivid colors, featuring exceptional transfer efficiency and binding affinity. FAQ Q: What is the protein concentration of this product in μg/μL? A: 0.1 - 0.4 μg/μL. Documents: Safety Data Sheet 20352_MSDS_HB250721 Manuals 20352_Manual_Ver.EN20250618 Related Blog From Unstained to Tricolor: The Evolution of Protein Markers for Smarter Western Blotting
