PNGase F is an amidase enzyme that is Cloned from Peace Station Eliza and mainly secreted by Neisseria meningitidis and other Gram-negative bacteria. Yeasen PNGase F is recombinantly expressed in yeast (specific activity: 100,000 U/mL), which can cleave high mannose, hybrid, and complex oligosaccharide glycoproteins linked by asparagine. The cleavage site of PNGase F is the amide bond between the inner N-acetylglucosamine (GlcNAc) and asparagine residue of glycoproteins, while converting the asparagine on the protein after enzymatic hydrolysis to aspartic acid. This product is tagged with His and is commonly used for complete deglycosylation of antibodies and related proteins. Feature High purity—No protease, glycosidase contamination, purity ≥95%. High Activity— 100,000 U/mL. Good compatibility—Can be used denaturing or no-denaturing conditions. Application Glycan Sequencing Proteomics Glycoprotein Analysis .Recombinant Glycoprotein Expression Specification English synonym PNGase F; N-Glycosidase F; N-Glycosidase F Source Yeast recombinant expression Molecular Weight 36 kDa Specific Activity 100,000 U/mL Storage buffer 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA, 50% Glycerol Unit Definition 1 unit of enzyme activity refers to the amount of enzyme required to remove more than 95% of the carbohydrates from 10 μg denatured RNase B in a 10 μL reaction system at 37°C within 1 hour. Components Components No. Name Component Composition 20407ES01 20407ES02 20407-A PNGase F PNGase F 15000 U 75000 U 20407-B1 Buffer 1 (10×) 5% SDS; 400 mM DTT 150 μL 750 μL 20407-B2 Buffer 2 (10×) 200 mM Tris, pH 7.5 300 μL 1500 μL 20407-B3 10% NP-40 10% NP-40 in MilliQ-H2O 300 μL 1500 μL Storage The product can be stored at -25 to -15℃ for one year. Figures Documents: Safety Data Sheet 20407_MSDS_HB260122 Manuals 20407_Manual_Ver.HB240524
