Super ECL Detection Reagent _ 36208ES
Super ECL Detection Reagent is designed to detect antibodies and associated antigens directly or indirectly labeled with horseradish peroxidase (HRP). The principle of Super ECL De tection Reagent is that, proteins or nucleic acids were transferred to the imprinted membrane after electrophoresis, and the target proteins on the membrane were bound by primary antibody and secondary antibody labeled with HRP, or the nucleic acids on the membrane were bound directly or indirectly by probes labeled with HRP. After washing the membrane, the ECL working solution prepared by the product was used to incubate the membrane at room temperature for several minutes. The imprinted membrane was wrapped with plastic wrap and fixed to the X-ray exposure Cassette. Then the X-ray film is pressed on the membrane in a darkroom and exposed for several seconds to several hours. After development and fixing, protein or nucleic acid bands can be clearly displayed on the X-ray film. This kit has a unique luminescent substrate system, Super ECL Detection Reagent is the most sensitive commercial fluorescent ECL detection reagent at present. Features High sensitivity and high signal-to-noise ratio, it can detect low pick-grade antigens. Shine quickly and brightly, the imprinted film band can be observed under fluorescent lamp. Long duration of luminescence, fluorescence can make X-ray film sensitive for more than 5 hours, especially suitable for detecting trace protein or nucleic acid. Higher dilution ratio of antibody (1:2000 ~ 1:10000) can be used to greatly save antibody. Applications Chemiluminescence ELISA Western Blot Dot Blot-DNA/RNA Southern blot-DNA Northern blot-RNA Specifications Recommended Antibody Concentrations primary antibody (liquid storage concentration 1mg/mL) : 1:1000-1:10000 secondary antibody (liquid storage concentration 1mg/mL) : 1:2000-1:10000 Signal Duration < 12 h Substrate Properties Chemical Substrate Substrate Type HRP (Horseradish Peroxidase) Substrate Sensitivity low pick-grade antigens Components Components No. Name 36208ES60 (100 mL) 36208ES76 (500 mL) 36208-A Super ECL Detection Reagent-Reagent A 50 mL 250 mL 36208-B Super ECL Detection Reagent-Reagent B 50 mL 250 mL Shipping and Storage The product is shipped with ice pack and can be stored at 2℃ ~ 8℃ for one year away from light. Figures Figure 1 Comparison of detection effect between Yeasen Super substrate and Pico substrate of the same level of other brandsYeasen ECL reagent has better use effect than other brands of the same level of products FAQ Q: Can this product detect antigens at the picogram level? A: Sure. This product is a highly sensitive type of test kit. Q: What is the concentration of the primary antibody used in this product? A: 1:1000 - 1:10000. Q: Can this product be applied to PVDF membranes? A: Yes, both NC membranes and PVDF membranes are applicable. Q: If the abundance of the sample target is high, can this product be used? A: Not recommended. This product is suitable for samples with low target abundance. Documents: Safety Data Sheet 36208_MSDS_HB220419_EN_1652931562504.PDF 36208_MSDS_HB220419_EN_1652931562549.PDF 36208_MSDS_HB220419_EN_1652931562813.PDF Manuals 36208_Manual_HB221103_EN.pdf Citations & References: [1] Wang Z, Lu Z, Lin S, et al. 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Super ECL Detection Reagent _ 36208ES
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