Salt Active UltraNuclease is a non-specific endonuclease which derived from Marine microorganisms and is expressed and purified in Escherichia coli (E. coli) by genetically engineered. This nonspecific, recombinant endonuclease has optimum activity at high salt concentration (500mM NaCl), which can improve efficiency and yield in various workflows. This kit uses the principle of double-antibody sandwich enzyme-linked immunosorbent assay (sandwich ELISA) to detect the residual Salt Active UltraNuclease in samples. First, add the Salt Active UltraNuclease standard and test sample to the Anti-Salt Active UltraNuclease coated microtiter strips (36719-A), then add the diluted biotin labeled Anti-UltraNuclease antibody (36719-C), and finally add Streptavidin-HRP (36719-D) to form an antibody + antigen + antibody-Biotin + SA-HRP complex. Subsequently, add TMB substrate (36719-H) into the complex to observe color reaction after washing the complex. TMB is converted into blue under the catalysis of HRP enzyme and finally converted into yellow in the presence of acid, and the shade of color is positively correlated with the amount of Salt Active UltraNuclease in the sample. The detection range of this kit is 0.047-3 ng/mL; the lower detection limit is 0.024 ng/mL. Feature Highly sensitive: Detecting as little as 0.024 ng/mL of Salt Active UltraNuclease residuals in in-process and final biological samples. Ensures accuracy: The Salt Active UltraNuclease residuals can be accurately detected with a recovery of 75%-125%. Specificity: Cooperate use with YEASEN Salt Active UltraNuclease and can accurately detect the residuals. Stable: The difference between lot-to-lot is low, the kit property is not impacted under 37℃ for 7 days. Easy signal collection: Using Biotin-Streptavidin system, the signal can be stably enlarged. Application Residual Salt Active UltraNuclease test in biological products Specification Sensitivity 0.024 ng/mL (range 0.047~3 ng/mL) Assay Time
