The Live Cell Analysis Kit contains SuperView™ 488 Caspase-3 Substrate and Ac-DEVD-CHO Caspase-3/7 Inhibitor. The SuperView™ 488 Caspase-3 Substrate provides an effective tool for detecting cell apoptosis based on Caspase-3/7 activity, suitable for fluorescence microscopy observation and flow cytometry analysis. Compared to other fluorescent substrates or inhibitors for Caspase analysis based on FLICA (Fluorochrome-Labeled Inhibitors of Caspases), the Substrate provided in this kit detects Caspase-3/7 activity without inhibiting the apoptosis process in intact cells. The Substrate consists of a fluorescent DNA dye coupled with the Caspase-3/7 DEVD recognition sequence. Initially non-fluorescent, the Substrate crosses the cell membrane into the cytoplasm. In apoptotic cells, Caspase-3/7 cleaves the Substrate, releasing a high-affinity DNA dye. This dye migrates to the nucleus, labels the DNA, and emits bright green fluorescence. The Substrate is bifunctional: it detects Caspase-3/7 activity while simultaneously visualizing morphological changes in the nucleus during apoptosis. SuperView™ staining is compatible with formaldehyde fixation and subsequent immunostaining experiments. Spectral Properties of SuperView™: Ex/Em = 500/530 nm (when bound to DNA) Features Dual-Function Detection: Simultaneously visualizes Caspase-3/7 activity and apoptotic nuclear morphology in live cells. Downstream Compatibility: Fully compatible with formaldehyde fixation and subsequent immunostaining protocols. Non-Inhibitory: Detects apoptosis without interfering with the cell death process. Components Components No. Name 40273ES25 (25 T) 40273ES60 (100 T) 40273-A SuperView 488 Caspase-3 Substrate, 0.2 mM in DMSO 125 µL 500 µL 40273-B Caspase-3 inhibitor Ac-DEVD-CHO, 2 mM in DMSO 20 µL 100 µL Figure Figure 1. Comparison of Flow Cytometry Scatter Plots (SSC-A vs. BL1-A) for Two Batches. Left (Lot 1) and right (Lot 2) panels show SSC-A vs. BL1-A fluorescence. The vertical line gates low (left) and high (right) BL1-A expressing cells. Lot 1: 81.9% low / 18.1% high. Lot 2: 85.7% low / 14.3% high. Both batches show similar distribution patterns.
