The mitochondrial membrane potential fluorescent probe JC-10 is an upgraded product of JC-1 and can also be used to detect changes in mitochondrial membrane potential. Although JC-1 has been widely used in many experiments, it has poor water solubility and will precipitate in aqueous buffer solutions even at a concentration of 1 μM. JC-10 has better water solubility and can replace JC-1 in experiments that require high concentrations of dye. In normal cells, JC-10 selectively aggregates in the mitochondrial matrix to form a reversible red fluorescent polymer (Ex=540 nm, Em=590 nm). In unhealthy mitochondria, due to the decrease or loss of membrane potential, JC-10 shifts from a polymeric form to a monomeric form in the cytoplasm, producing green fluorescence (Ex=490 nm, Em=525 nm). JC-10 can not only be used for qualitative detection, as the color change can directly reflect the change in mitochondrial membrane potential, but also for quantitative detection, as the degree of mitochondrial depolarization can be measured by the ratio of red to green fluorescence intensity. These two colors changes can be detected by standard filters on a flow cytometer, with green fluorescence analyzed by the FL1 channel and red fluorescence by the FL2 channel. In addition to flow cytometry, it can also be used for fluorescent imaging and fluorescence microplate detection platforms. JC-10 performs better than JC-1 in some cell lines. However, the performance of JC-10 is highly cell-dependent. Features Suitable for a variety of cell types. Highly affinity to mitochondria. Better water solubility Applications This product can be used for detection of mitochondrial membrane potential in cells, tissues, or purified mitochondria Specifications Chemical name 5,6 -Dichloro-1,1’,3,3’-tetraethyl-imidacarbocyanine iodide CAS NO. 5563-28-0 Formula C25H27Cl2IN4 Molecular Weight 583.34 Purity ＞95%（HPLC） Appearance Red powder Solubility Soluble in DMSO Fluorescent spectrum Monomer form：Ex=490 nm, Em=525 nm J-aggregate form：Ex=540 nm, Em=590 nm Components Components No. Name 40707ES03 40707ES08 40707 JC-10 Mitochondrial Membrane Potential Fluorescent Probe 1 mg (2 mg/mL) 5 mg Shipping and Storage Transported with wet ice. Store the powder at -20°C in a dry, light-protected environment for at least one year. Aliquot the storage solution into single-use volumes and store at -20°C in a dry, light-protected environment to avoid repeated freeze-thaw cycles, which is effective for about one year. Documents: Safety Data Sheet 40707ES03_MSDS_CN20250116 40707ES08_MSDS_CN20250116 Manuals 40707_Manual_Ver.CN20250116 Citations & References: [1] Li W, Duan A, Xing Y, Xu L, Yang J. Transcription-Based Multidimensional Regulation of Fatty Acid Metabolism by HIF1α in Renal Tubules. Front Cell Dev Biol. 2021;9:690079. Published 2021 Jul 2. doi:10.3389/fcell.2021.690079(IF:6.684) [2] Xu Y, Zhang Y, Xu Y, et al. Activation of CD137 signaling promotes macrophage apoptosis dependent on p38 MAPK pathway-mediated mitochondrial fission. Int J Biochem Cell Biol. 2021;136:106003. doi:10.1016/j.biocel.2021.106003(IF:5.085) [3] Xu S, Yang X, Qian Y, Luo Q, Song Y, Xiao Q. Analysis of serum levels of organochlorine pesticides and related factors in Parkinson's disease. Neurotoxicology. 2022;88:216-223. doi:10.1016/j.neuro.2021.12.001(IF:4.294)
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