Booster DNA/RNA Transfection Reagent is an advanced nucleic acid transfection reagent developed based on novel polymeric materials. Its unique molecular design significantly reduces cytotoxicity and features a smart anti-enzymatic degradation mechanism that protects nucleic acids from intracellular enzymatic degradation during delivery, thereby maximizing transfection efficiency. This reagent is suitable for various types of nucleic acids including DNA, siRNA, miRNA, mRNA, and ASO, and has been validated for efficient transfection in cell lines such as 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW264.7, HCT116, as well as primary cells, providing reliable tools for gene research. Unique Mechanism Enhances Transfection Efficiency at Every Step. Hieff Trans™ Booster is a next-generation polymeric transfection reagent. By utilizing a novel polymeric formulation—identified through our AI-integrated high-throughput screening, it achieves significantly higher delivery efficiency in challenging models—such as primary neurons, T-cells, and suspension cells—while ensuring exceptional cell viability. Its unique mechanism enhances transfection efficiency at every step, delivering higher success rates for your transfection experiments! 1) High Loading Capacity → Dual mechanism: charge interaction + polymer chain entanglement; Higher loading capacity than traditional charge-interaction methods. 2) Efficient Cellular Uptake → Membrane translocation; Faster than conventional endocytosis pathways. 3) Non-Damaging Release → Rapid polymer degradation by intracellular enzymes/reducing GSH; Zero-damage release of nucleic acids. 4) Enhanced Safety → Rapid polymer degradation property; Lower toxicity than traditional lipid-based transfection reagents. Features Low Cytotoxicity: The novel polymer replaces traditional liposomes to reduce cytotoxicity and improve cell viability. Fast Release and Higher Efficiency: Fast release minimizes intracellular nucleic acid degradation, achieving >90% transfection efficiency (measured by flow cytometry). Premium Options for Difficult-to-transfect cell lines: Sensitive and primary cells. Proven Performance, Broad Cell Line Coverage: Achieves high efficiency in 200+ various cell lines including 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW264.7, and HCT116 and primary cells, with consistent and reproducible results. One Reagent, All Nucleic Acids: Delivers DNA, siRNA, miRNA, mRNA, and ASO with high efficiency—even up to 15 kb fragments. Components Components No. Name 40801ES01 40801ES03 40801ES04 40801-A Booster DNA/RNA Transfection Reagent 100 μL 0.75 mL 1.5 mL 40801-B Transfection Enhancer 100 μL 0.75 mL 1.5 mL Application Cell Transfection; DNA Transfection; mRNA/siRNA Transfection; Figures 1) High-Efficiency DNA Transfection Figure 1. DNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior DNA transfection efficiency of Booster. 2) High-Efficiency mRNA Transfection Figure 2. mRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior mRNA transfection efficiency of Booster. 3) High-Efficiency siRNA Transfection Figure 3. siRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior siRNA transfection efficiency of Booster. Customer Feedback 1) High-Efficiency Primary Dermal Fibroblasts Transfection Figure 4. Plasmid EGFR DNA transfection in primary dermal fibroblasts using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster. 2) High-Efficiency Porcine Alveolar Macrophages Transfection Figure 5. DNA transfection in primary Porcine Alveolar Macrophages (PAMs) using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster. Try it for free! Get a free sample of Hieff Trans™ Booster and validate its performance in your own lab.👉 [Request Sample Now]. Worldwide Shipping. Related Blog Beyond Liposomes: The Next Generation Booster DNA/RNA Transfection Reagent siRNA Transfection？ The novel reagent here.
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