PEI 40000 is a highly charged cationic polymer with a molecular weight of 40,000 that binds negatively charged nucleic acid molecules very easily, forming a complex and allowing the complex to enter cells. PEI 40000 is a transient transfection reagent with low cytotoxicity, high transfection efficiency, and high gene expression efficiency in cells such as HEK293 and CHO. PEI 40000 has many advantages over PEI 25000 Transfection Reagent. include: 1. PEI 40000 is easy to dissolve and can be directly dissolved in water. PEI 25000 needs to first adjust the water to weak acid to help it dissolve, and then use NaOH to adjust the pH to neutral. 2. PEI 40000 is easy to operate, easier to use, and has a better transfection effect than PEI 25000; 3. PEI 25000 contains 4-11% propionyl residues, which can prevent the polymer backbone from binding to DNA. Compared to PEI 25000, PEI 40000 is a complete shed construction, so its performance is consistently efficient. This product is an instant type, which dissolves quickly and is easy to prepare. Feature High Transfection Efficiency: Ideal for DNA transfection; transfection efficiency can reach over 80% when cell density is at 70%–80%. Broad Applications: Suitable for both adherent and suspension cells, and compatible with both transient and stable transfection. Low Cytotoxicity: Transfected cells maintain good morphology and express high levels of target proteins. Easy Protocol: Solutions can be prepared directly with distilled water, and complexes can be added directly to serum-containing media. Cost-Effective: At the same volume/specifications, the price is one-third of the competitor's poly product, making it ideal for large-scale transfection. Application Cell Transfection Specification Name Polyethylenimine Linear (PEI) 40000 CAS No. 49533-93-7 Molecular formula (CH₂CH₂NH)n Molecular weight 40,000 Solubility Soluble in water, insoluble in organic solvents: benzene, ether and acetone Structure Form White or off-white solid Serum Compatible Yes Cell Type Established Cell Lines Sample Type Plasmid DNA Transfection Technique PEI-Based Transfection Components Name 40816ES01 40816ES02 40816ES03 40816ES10 Polyethylenimine Linear(PEI) MW40000 (Rapid Dissolving) 5 mg 100 mg 1 g 10 g Shipping and Storage The product is shipped at room temperature and the powder can be stored at 2-8ºC for two years. The stock solution is stored at 2-8 ºC for 3 months. Figures 1. Demonstration of Transfection Effect Figure 1. The transfection efficiency of Polyethylenimine Linear (PEI) MW40000 Reagent was shown above. Polyethylenimine Linear (PEI) MW40000 Reagents were used to transfect the HEK293 cell line and CHO-K1 cell line in a 96-well format. GFP expression was analyzed 48 hours posttransfection. 2. Validated Cell Lines Product Name Polyethylenimine Linear (PEI) MW40000 (Rapid Lysis) Validated Cell Lines COS-1 CHO-K1 HEK293 HepG2 COS-7 Hela HEK293T NIH-3T3 Cited from "TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell . 2022 Mar 17;185(6):980-994.e15. doi: 10.1016/j.cell.2022.02.010. " Documents: Safety Data Sheet 40816_MSDS_HB260807 Manuals 40816_Manual_HB20260807 FAQs: Q: Can 40816 be made into 10× mother liquor and stored at -20℃? A: It is recommended to store in accordance with the storage conditions of 2-8 ℃ in the instructions. Do not store at -20°C, use hydrochloric acid to make the pH slightly acidic, pH 6.8, and then store at -20°C, However, the storage time cannot exceed one year. When using it again, take it out and measure the pH. If the pH is between 6.8 and 6.9, it can be used directly. Otherwise, the pH needs to be adjusted, and it cannot be frozen again to avoid repeated freezing and thawing. It is more troublesome and may affect the transfection efficiency, so it is not recommended. Citations & References: [1] Li X, Li J, Li G, et al. Engineered immunosuppressive dendritic cells protect against cardiac remodelling. Nature. 2026;10346. doi:10.1038/s41569-026-01294-8 (IF: 50.5) [2] Luo J, Yang Q, Zhang X, et al. TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell. 2022;185(6):980-994.e15. doi:10.1016/j.cell.2022.02.010(IF:41.584) [3] Chen Y, Luo R, Li J, et al. Intrinsic Radical Species Scavenging Activities of Tea Polyphenols Nanoparticles Block Pyroptosis in Endotoxin-Induced Sepsis [published correction appears in ACS Nano. 2022 Mar 3;:]. ACS Nano. 2022;16(2):2429-2441. doi:10.1021/acsnano.1c08913(IF:15.881) [4] Chen ZH, Yan SM, Chen XX, et al. The genomic architecture of EBV and infected gastric tissue from precursor lesions to carcinoma. Genome Med. 2021;13(1):146. Published 2021 Sep 7. doi:10.1186/s13073-021-00963-2(IF:11.117) [5] Huang G, Liu D, Wang W, et al. High-resolution structures of human Nav1.7 reveal gating modulation through α-π helical transition of S6IV. Cell Rep. 2022;39(4):110735. doi:10.1016/j.celrep.2022.110735(IF:9.423) [6] Tian X, Liu L, Jiang W, Zhang H, Liu W, Li J. Potent and Persistent Antibody Response in COVID-19 Recovered Patients. Front Immunol. 2021;12:659041. Published 2021 May 28. doi:10.3389/fimmu.2021.659041(IF:7.561) [7] Lin J, Chen Z, Yang L, et al. Cas9/AAV9-Mediated Somatic Mutagenesis Uncovered the Cell-Autonomous Role of Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 in Murine Cardiomyocyte Maturation. Front Cell Dev Biol. 2022;10:864516. Published 2022 Apr 1. doi:10.3389/fcell.2022.864516(IF:6.684) [8] Nian F, Qian Y, Xu F, Yang M, Wang H, Zhang Z. LDHA promotes osteoblast differentiation through histone lactylation. Biochem Biophys Res Commun. 2022;615:31-35. doi:10.1016/j.bbrc.2022.05.028(IF:3.575) Related Blog 1. Literature Insight | Immunosuppressive Dendritic Cells in Cardiac Remodeling 2. Beyond Liposomes: The Next Generation Booster DNA/RNA Transfection 3. Next-Gen siRNA Delivery: Achieve >90% Knockdown Efficiency with Lower Reagent Usage —Even in Hard-to-Transfect Cells.
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