Polyethylenimine Linear(PEI) MW 25000 _ 40815ES

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Linearized Polyethyleneimine PEI 25000 Transfection Reagent is a highly charged cationic polymer that easily binds negatively charged nucleic acid molecules, forms a complex, and a llows the complex to enter the cell. The transfection reagent is a transient transfection reagent with low cytotoxicity, high transfection efficiency, and high gene expression efficiency in cells such as HEK293 and CHO. It has been verified that the linear PEI transfection reagent is widely applicable to a variety of cell lines including HEK-293, HEK293T, CHO-K1, COS-1, COS-7, NIH/3T3, Sf9, HepG2 and Hela cells, etc. The reagent is compatible with serum-containing media and can efficiently introduce nucleic acids into cells. Specification Name Polyethylenimine Linear (PEI) MW25000 CAS No. 9002-98-6, 26913-06-4 Molecular formula (CH2CH2NH)n Molecular weight 25,000 Appearance white or light yellow powder Melting Point 73~75 ℃ Solubility Soluble in hot water, cold water with low pH, methanol and ethanol. Insoluble in benzene, ether and acetone Structure Shipping and Storage The product is shipped at room temperature and the powder can be stored at 2-8ºC for two years. The stock solution is stored at 2-8 ºC for 3 months. Cautions 1) After the prepared PEI solution is taken out from -20 ºC and thawed, it can be stored in a 4 ºC refrigerator and must not be re-frozen.2) For most cells, 3.0 μL of PEI transfection reagent per 1 μg of DNA can achieve high transfection efficiency. You can also try to use 1.5~4 μL volume of linear PEI transfection reagent per 1 μg DNA for optimization.3) For your safety and health, please wear a lab coat, disposable gloves and a fume hood for operation.4) This product is only for scientific research purposes, not for human use. Stock solution Preparation (1 mg/mL)1. MaterialsPEI 25000, Milli-Q® water/water for injection (WFI) or similar biological grade water, 12 mol/L hydrochloric acid (HCl), 10 mol/L sodium hydroxide (NaOH), disposable 0.1~0.2 μm PES vacuum sterile filter, sterile HDPE or polypropylene storage bottle.2. Prepare stock solution (1 mg/mL)1) In a 1 L glass beaker, add 1 g of PEI 25000 powder into 900 mL of Milli-Q® ultrapure water or other equivalent biological water, and stir evenly on a magnetic stirrer to produce a small vortex.2) Add hydrochloric acid (12 mol/L) dropwise while stirring to adjust the pH until pH Show more