Coelenterazine f is a derivative of native coelenterazine and serves as a substrate for aequorin (jellyfish luminescent protein). The structural difference between coelenterazine f and native coelenterazine lies in the substitution of the hydroxyl group on the phenol ring at the R-1 position with a fluorine (F) atom. When coelenterazine f and aequorin are oxidized, they are activated to form a metastable complex of coelenterazine. The formation of this complex occurs very rapidly, whereas the formation of the native coelenterazine–aequorin complex is much slower (only 40% of native coelenterazine reacts within 60 minutes). Compared to the total photon energy produced by the native coelenterazine–aequorin complex, this complex generates only 80% of the photon energy. Upon contact with Ca2+, coelenterazine f can rapidly and efficiently emit light, with an energy output that is 20 times stronger than that of native coelenterazine. Additionally, coelenterazine f exhibits the best cell permeability. When extremely high Ca2+detection sensitivity is required for experiments involving aequorin regeneration, this substrate is highly recommended. Features No radiation, almost harmless to living organisms. Bioluminescence, no excitation light source. So sensitive, you can detect it in a few hundred cells. Good penetration, 3-4cm tissue depth can still be detected. High signal-to-noise ratio, strong fluorescence signal and good anti-interference. Applications In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement. To test the effect of the administration on tumor growth or metastasis. The localization and distribution of foreign cells in animals were detected. The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression. Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo. Specifications English synonym Coelenterazine f CAS NO. 123437-16-1 Formula C26H20N3O2 Molecular weight 425.46 g/mol Appearance Yellow to brown powder Solubility Soluble in methanol and ethanol, insoluble in DMSO Purity ≥ 95% Components Components No. Name 40908ES02 40908ES03 40906 Coelenterazine f 1×500 μg 2×500 μg Shipping and Storage -20℃ keep away from light and dry storage, preferably stored in an inert gas environment, avoid contact with air; long-term storage at -70℃. Valid for 2 years. Figures Documents: 40908_MSDS_HB241231 40908_Manuals_Ver.EN20241231 Citations & References:
