Interleukin 12 (IL-12) is a potent regulator of cell-mediated immune responses, inducing IFN-γ-production by NK cells and T cells.IL12 is produced by activated monocytes/macrophages, B lymphocytes, and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+, and CD8+ cells and induces the development of IFN-γ-producing Th1 cells. Recombinant Human IL-12 is a disulfide-linked p35 subunit and p40 subunit. This Recombinant Human IL-12 is supplied as Fluids, high activity, high purity, low endotoxin, NO labeling. Product Properties Synonyms IL12; p70; Interleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF Species Human Source HEK293 Ceslls Sequence） IL-12 p40 (Ile23-Ser328), IL-12 p35 (Arg23-Ser219) Accession P29460 (IL-12p40), P29459 (IL-12p35) Tag NO Predicted molecularmass 22.5kDa+34.7kDa Endotoxin 95%, determined by SDS-PAGE Activity Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts.The EC50 for this effect is 4-40 pg/mL Formulation Dissolved in sterile PBS buffer. Appearance Fluids Shipping and Storage -85~-65℃ storage, valid for 1 year after receipt. Avoid repeated freezing and thawing, centrifuge before opening the vial. It is recommended to aliquot for one experimental use, after aliquoting, freeze at -80℃. Figure Figure 1.Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The EC50 for this effect is 4-40 pg/mL. Figure 2.2 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining. Cautions 1. For your safety and health, please wear lab coat and disposable gloves. 2. This product is for scientific research purposes only.
