Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses, inducing the production of IFN-γ by NK and T cells.IL12 is produced by activated monocytes/macrophages, B-lymphocytes, and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+, and CD8+ cells and induces the development of IFN-γ-producing Th1 cells. Recombinant Human IL-12 is a disulfide-linked p35 subunit and p40 subunit. This Recombinant Human IL-12 is supplied as a lyophilized powder with high activity, high purity, low endotoxin, and no redundant labeling. Specification Synonyms IL12; p70; Interleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF Species Human Source CHO Cells Sequence Recombinant human IL-12 is composed of P40(Ile23-Ser328) andP35(Arg23-Ser219) subunits,which linked by GS-linker（GSGSSRGGSGSGGSGGGGSK） Accession P29460(IL12P40), P29459(IL12P35) Tag Not Molecular Weight Predicted molecularmass：59 kDa Endotoxin ≤10 EU/mg by the LAL method Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) Activity Measured by its ability to induce Interferon gamma secretion by human naturalkiller lymphoma NK-92 cells. The ED50 for this effect is ＜0.5 ng/mL. Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitoland 0.01% Tween 80, pH 7.4 Appearance Lyophilized powder Storage -25 ~ -15 ° C storage, from the date of receipt of goods, the expiration date of 1 year. After re-solution, store at 2 ~8 °C, 7-10 days expiration date. After re-dissolution, -85 ~ -65 ° C storage, 6 months expiration date. Compounding Methods Use sterile deionized water to reconstitute freeze-dried proteins. It is recommended that proteins be divided for first use to avoid repeated freeze-thawing. Cautions 1. Please operate with lab coats and disposable gloves,for your safety. 2. This product is for research use only.
