IL-33 , a 30 kDa pro-inflammatory protein.IL-33 is constitutively expressed in smooth muscle and airway epithelium. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes after IL-1 alpha or IL-1 beta stimulation. The C-terminal fragment of mature IL-33 binds and triggers signaling through mast cell IL-1 R4/ST2L, an orphan receptor chronically involved in augmenting Th2 cell responses. Subsequently, IL-33 and ST2L bind to IL-1R AcP to form a ternary signaling complex. In vitro stimulation of Th2 polarized lymphocytes with mature IL-33 induces IL-5 and IL-13 secretion. In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues. Full-length and mature human IL-33 shares 52-58% amino acid sequence identity with mouse and rat IL-33. This product is available as Fluids, high activity, high purity, low endotoxin, NO labeling. Product Properties Synonyms IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin -33 Species Human Source HEK293 Ceslls Ceslls Sequence Ser112-Thr270 Accession O95760.1 Tag NO Predicted molecularmass 18.0 kDa Endotoxin 95%, determined by SDS-PAGE Activity Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The EC50 for this effect is 0.05-0.20 ng/mL Formulation Dissolved in sterile PBS buffer. Appearance Fluids Shipping and Storage -85~-65℃ storage, valid for 1 year after receipt. Avoid repeated freezing and thawing, centrifuge before opening the vial. It is recommended to aliquot for one experimental use, after aliquoting, freeze at -80℃. Figure Figure 1.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The EC50 for this effect is 0.05-0.20 ng/mL. Figure 2.2 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining. Cautions 1. For your safety and health, please wear lab coat and disposable gloves. 2. This product is for scientific research purposes only.
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