Hieff™ Super Bst DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I and has been genetically modified to remove its 5’-3’ exonuclease activity. This product possesses strong 5’-3’DNA polymerase activity, strand displacement activity, and dUTP tolerance, making it more suitable for contamination prevention in isothermal amplification reactions such as LAMP, CPA, etc. Compared to Bst Plus DNA Polymerase, Hieff™ Super Bst DNA Polymerase has improved the thermal stability of the Bst enzyme, which helps to enhance the amplification performance of the Bst enzyme. This enzyme is suitable for low-template and high-temperature LAMP reaction systems, effectively improving the specificity of detection and reducing the detection time. The high-temperature resistance characteristic is also applicable for the development of heat-resistant Bst, further enhancing the specificity of detection. Features Thermophilic DNA polymerase with strong strand displacement activity Applications LAMP, CPA, RCA etc. High Temperature LAMP/RT-LAMP, Over 70℃ Specifications Polymerase Bst DNA Polymerase Activity Definition 1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C. Solution Components 10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃. Components Components No. Name 14410ES10 20 KU 14410ES50 100 KU 14410ES60 200 KU 14410 Hieff ™ Super Bst DNA Polymerase(2,000 U/μL) 10 μL 50 μL 100 μL Figure Figure 1. RT-LAMP testing with Hieff Super BST at 70℃. Figure 2. Perfomance of the mutants of Bst DNA polymerase in LAMP asays. (a) Perormance of the mutants of Bst DNA polymerase in LAMP assays under diferent temperatures. (b) Compared with Bst DNA polymerase of NEB (green), mutants M2 (orange) and M3 (red) exhbited greatly increased activity under different temperatures for diferent targets. (c) Performance of M2 in the fale positive experiment. (d) Performance of M3 in the false positive experiment. (e) Perfomance of the mutants of BST in the thermal acceleration experiment at 50 ℃ for 63 days. Shipping and Storage The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 years. Please avoid repeated freeze-thaw. Document: Safety Data Sheet 14410ES_MSDS_HB250211 Manuals 14410_Manual_Ver. HB250208 Publications FADS-Based Directed Evolution of a Robust Bst DNA Polymerase Adapting High-Temperature Loop-Mediated Isothermal Amplification (HT-LAMP) ACS Catal. 2025, 15, 7, 5391–5402 Related blog: Yeasen Groundbreaking Milestone in High Temperature Bst DNA Polymerase Evolution for Eliminating False Positive Issues in LAMP Assays
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