Tumor necrosis factor alpha (TNF-α), also known as cachectin, is a cachectin, is a polypeptide cytokine produced by monocytes and macrophages. TNFα receptor signaling can simultaneously activate caspase 8, the transcription factor, NF-κB and the kinase, JNK. Human TNF-α shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. Inflammation is associated with insulin resistance, and TNF-α and IL-6 may affect glucose uptake. A wide variety of evidence has pointed to a critical role of TNF-α in tumour proliferation, migration, invasion and angiogenesis. Product Properties Synonyms Tumor Necrosis Factor, TNFA, TNFSF2, Cachectin, Cytotoxin, Differentiation-inducing factor, DIF Accession P16599 GeneID 24835 Source E.coli-derived rat TNF-α/TNFSF2 protein, Leu80-Leu235. Molecular Weight Approximately 17.2 kDa. AA Sequence LRSSSQNSSD KPVAHVVANH QAEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLIYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV SLLSAIKSPC PKDTPEGAEL KPWYEPMYLG GVFQLEKGDL LSAEVNLPKY LDITESGQVY FGVIAL Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 98% by SDS-PAGE and HPLC analyses. Biological Activity The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D. Fully biologically active when compared to standard. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2, 150 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20 ℃ for 1 year. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions Avoid repeated freeze-thaw cycles. For your safety and health, please wear lab coats and disposable gloves for operation. For research use only!
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