Specifications Source Recombinant Human IL-18BP Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Thr31-Gly194.[uniprot | O95998-2] Molecular Weight The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Human IL-18 at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 36.5ng/ml determined by ELISA SPR Data: Human IL-18BP, hFc Tag captured on Protein A chip, can bind Human IL-18 with an affinity constant of 0.04nM as determined in a SPR assay Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
