Activin A is a member of the TGF-β family and exhibits a wide range of biological activities, including the regulation of cell proliferation and differentiation, as well as the promotion of neuronal survival. Elevated levels of Activin A in human colorectal cancer and postmenopausal women are associated with colorectal cancer and breast cancer, respectively. The biological activity of Activin A can be neutralized by inhibin and the diffusible TGF-β antagonist follistatin. Activin A binds to two forms of Activin Receptor Type I (ActR-I-A and ActR-I-B) and two forms of Type II receptors (ActR-II-A and ActR-II-B). Activins are homodimers or heterodimers of various β subunits. They are produced as precursor proteins with an N-terminal propeptide, which is cleaved to release the C-terminal bioactive ligand. Product Information Product Name Product Number Specification HiActiTM Recombinant Human Activin A Protein,His Tag 91717ES10 10 μg 91717ES50 50 μg 91717ES72 250 μg Performance Parameters Synonyms Activin A, activin AB alpha polypeptide, erythroid differentiation factor, Erythroid differentiation protein, FSH-releasing protein, inhibin beta A chain Species Human Sequence Gly311-Ser426 Accession P08476 Tag N-His Expression System E.coli Molecular Weight Theoretical MW is approximately 20.0 kDa Purity > 95% as determined by SDS-PAGE Endotoxin Less than 1.0 EU per μg by the LAL method Formulation 20mM NaHCO3, pH8.3 Appearance Liquid Bioactivity Determined by cell assay, Activin A inhibits the proliferation of mouse plasmacytoma cells MPC-11, with an ED50 of approximately 4ng/ml Storage Store at -25 ~ -15°C. Valid for 1 year from the date of receipt. Avoid repeated freeze-thaw cycles. Centrifuge before opening the vial. It is recommended to aliquot into single-use portions for each experiment. Product Features High activity, high purity, low endotoxin, high stability. Application Examples Figure 1. SDS-PAGE Human Activin A on SDS-PAGE under reduced (R) condition. The purity is greater than 95%. Figure 2. Cell Based Assay Determined by cell-based assay, Activin A protein inhibits the proliferation of mouse plasmacytoma cells MPC-11, with an ED50 of approximately 4 ng/mL. Documents: Manuals 91717_Manual_HB20260408
