Description Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup, where BMP2-7 belongs to TGF- β Superfamily. It plays a dominant role in embryonic dorsal ventral pattern, organogenesis, limb bud formation, and bone formation and regeneration. Mature human BMP-2 exhibits 100% aa sequence identity with mouse and rat BMP-2. It has 85% sequence identity with human BMP-4 and less than 51% sequence identity with other BMPs. Normal bone formation is a carefully regulated long-term process that involves the continuous expression of growth regulatory factors by osteoblasts during proliferation and final differentiation. Due to the ordered sequence of gene expression in osteoblasts indicating cascading effects, BMP-2 can initiate and maintain this effect. BMP-2 also promotes the maintenance and repair of colonic epithelium, inhibits the synthesis and release of dopamine in neurons, induces apoptosis in medulloblastoma cells, and is essential for myocardial contractility. Product information Synonyms BDA2, BMP2, BMP-2, BMP-2A, SSFSC Uniprot No. P12643 GeneID 650 Source E.coli-derived human BMP-2, Gln283-Arg396, with an N-terminal Met. Molecular Weight Approximately 26.0 kDa. Tag None Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >95 % by SDS-PAGE and HPLC analyses. Biological Activity The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/mL, corresponding to a specific activity of > 5.0 × 103 IU/mg. Fully biologically active when compared to standard. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM sodium citrate, pH 3.5. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions For your safety and health, please wear lab coats and disposable gloves for operation. For research use only! Documents: Safety Data Sheet Manuals 92051_Manual_Ver.EN20260303.pdf
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