Background BMP-7 is a member of the TGF-β superfamily which is supposed to be one of the most potent anabolic factors of chondrocytes. The growth factor domain of human BMP-7 shares 98% aa sequence identity with mouse and rat BMP-7. BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development, inhibits the branching of prostate epithelium, and antagonizes epithelial-mesenchymal transition (EMT). Bone morphogenetic protein (BMP) signaling regulates a range of cellular processes and plays an important role in the specification and patterning of the early embryo. BMP-7 expression is known to be protective for renal damage during diabetic nephropathy and disappears early during the progression of diabetic nephropathy. The clinical application of BMP7 is based on its neuroprotective role in stroke animal models. Product Properties Synonyms BMP7, BMP-7, bone morphogenetic protein 7, Eptotermin alfa, OP-1, Osteogenic protein 1 Accession P18075 GeneID 655 Source E.coli-derived human BMP-7, Ser293-His431. Molecular Weight Approximately 15.7 kDa. AA Sequence STGSKQRSQN RSKTPKNQEA LRMANVAENS SSDQRQACKK HELYVSFRDL GWQDWIIAPE GYAAYYCEGE CAFPLNSYMN ATNHAIVQTL VHFINPETVP KPCCAPTQLN AISVLYFDDS SNVILKKYRN MVVRACGCH Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >95% by SDS-PAGE and HPLC analyses. Biological Activity Testing in progress Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30% acetonitrile, 0.1% TFA.. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20 ℃ for 1 year. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions Avoid repeated freeze-thaw cycles. For your safety and health, please wear lab coats and disposable gloves for operation. For research use only.
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