Galectin-1, a polypeptidic factor that can have major effects on cell growth and apoptosis, is an important regulator of leukocyte function and tumor angiogenesis. Galectin-1 belongs to a family of 15 structurally related β-galactoside binding proteins that are able to control a variety of cellular events, including cell cycle regulation, adhesion, proliferation, and apoptosis. Human Galectin-1 shares 88% aa sequence identity with mouse, equine and ovine, 90% with rat, and 87% with bovine and porcine Galectin-1. Recombinant human galectin-1 to three porphyrin compounds: Zn-porphyrin (ZnTPPS). Mn-porphyrin and Au-porphyrin. These compounds are widely applied in the photodynamic therapy of cancer. Galectin-1 can also modulate cell-cell and cell-matrix interactions. Product Properties Synonyms Beta-galactoside-binding lectin L-14-I, BHL, DKFZp686E23103, GAL1, gal-1, Galaptin, galectin 1, Galectin1, Galectin-1, GBP, HBL, HLBP14, HPL, L-14, Lactose-binding lectin 1, LGALS1 Accession P09382 GeneID 3956 Source E.coli-derived Human Galectin-1, Ala2-Asp135. Molecular Weight Approximately 14.6 kDa. AA Sequence ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human blood monocytes is in a concentration range of 1.0-10 ng/mL. Fully biologically active when compared to standard. Endotoxin < 0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 1 mM DTT. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20 ℃ for 1 year. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions Avoid repeated freeze-thaw cycles. For your safety and health, please wear lab coats and disposable gloves for operation. For research use only!
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