R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3. Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids . Product Properties Synonyms R-Spondin 1;Roof Plate-specific Spondin 1；Cristin3; R-spondin-1 Source Expressed in E. coli. Molecular Weight Approximately 20.9kDa, a single non-glycosylated polypeptide chain containing 189 amino acid Accession Q9Z132: Ser21-Gly209 Biological Activity Fully biologically active when compared to standard. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5ng/mL recombinant mouse Wnt-3a. Purity > 96% by SDS-PAGE. Endotoxin ＜0.01EU/μg protein as determined by LAL method. Formulation Lyophilized from a 0.2mm filtered solution in 20mM PB, 150mM NaCl, pH 7.4. Reconstitution Before use this product, please read the direction below carefully. 1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. 2.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 1 month, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
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