R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt regulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all of which contain a Frizzled-like structural domain and a platelet-responsive protein structural domain.Rspo-1 is expressed in certain regions of the developing central nervous system, as well as the adrenal glands, ovaries, testes, thyroid gland, and trachea.Rspo can interact with the Wnt/β-catenin signaling pathway in a manner that stimulates the Frizzled/LRP6 receptor complex interaction. With the expanding field of organoids, R-Spondin 1 is widely used in organoid cell cultures as an essential component to promote growth and survival of 3D organoids.. Specification Synonyms R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1 Species Mouse Source HEK293 Sequence Ser21-Gln265 Accession Q9Z132 Tag C-His Molecular Weight The protein has a predicted MW of 28.3 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result. Endotoxin < 1 EU/μg by the LAL method. Purity > 95% as determined by Bis-Tris PAGE Activity ELISA Data: Immobilized Mouse R spondin 1, His Tag at 5 μg/ml (100 μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11 μg/ml determined by ELISA. Cell Based Assay: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 4 - 20 ng/mL in the presence of 5 ng/mL recombinant Human Wnt3a . Formulation Lyophilized from 0.22 μm filtered solution in 20 mM MES, 150 mM NaCl, 200 mM Arginine (pH 5.5). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization. Appearance Lyophilized powder Storage -25 ~ -15 ° C storage, 1 year expiration date after receipt. After re-solution, store at 2 ~8 °C, 7 days expiration date. After re-dissolving, store at -85~-65°C, 3 months expiration date. Compounding Methods Centrifuge before uncapping. It is recommended to reconstitute to a concentration of more than 100 μg/ml. lyophilized proteins are dissolved in 20 mM MES, 150 mM NaCl, 200 mM Arginine (pH 5.5). Cautions 1. For your safety and health, please wear lab coat and disposable gloves. 2. This product is for scientific research purposes only. Documents: Safety Data Sheet Manuals 92295-Manual-Ver.EN20260303
