Product Information Product Name Cat# Size Price（¥） Recombinant Human Sonic Hedgehog N-Terminus (Human SHH) 92566ES08 5 μg 815.00 92566ES60 100 μg 7335.00 92566ES76 500 μg 19055.00 Product Description Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. The Hedgehog (Hh) family of secreted proteins plays an essential role in metazoan development, and defects in the Hh pathway lead to congenital abnormalities such as holoprosencephaly and certain forms of cancer. Shh is the most widely expressed of the many ligands, and much of what is known about vertebrate Hh signaling stems from studies on Shh. Though similar in many ways to the DrosophilaHh pathway, several features of vertebrate Hh signaling make it unique. Product Properties Synonyms HHG1, HHG-1, HLP3, HPE3, MCOPCB5 Accession Q15465 GeneID 6469 Source E.coli-derived human Shh protein, Cly25-Gly197, with an N-terminal Met. Molecular Weight Approximately 19.8 kDa. AA Sequence IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 98% by SDS-PAGE and HPLC analyses. Biological Activity The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/mL, corresponding to a specific activity of > 1.0 × 103 IU/mg. Fully biologically active when compared to standard. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!
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