Biotinylated Human CD3E/CD3 epsilon 1-27PEptide Protein, hFc-Avi Tag

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Specifications Source Biotinylated Human CD3E/CD3 epsilon 1-27 peptide Protein is expressed from HEK293 with hFc tag and Avi Tag at the C-terminal. It contains Asp23-Thr48.[Accessi on | P07766] Molecular Weight The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Anti-CD3 Antibody, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD3E 1-27 peptide, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only. Show more