Laminin 521 is a defined matrix for feeder-free culture of human embryonic (hES) and induced pluripotent (hiPS) stem cells. When used in combination with defined maintenance media, Laminin 521 reliably facilitates self-renewal of human hES and hiPS cells without spontaneous differentiation. Serving as a biologically pertinent extracellular matrix for hPSCs, essential for their growth and maintenance of pluripotency in humans, Laminin 521 demonstrates robust compatibility with various commercial stem cell culture media. It effectively promotes the attachment and expansion of human pluripotent stem cells (hPSCs) whether in single-cell or small colony formats. The function of Laminin 521 is mediated through its binding to cell receptors, specifically a6β1 integrin. Laminin 521 significantly enhances the differentiation, polarization, and organization of various cell types, including neurons, hepatocytes, cardiomyocytes, retinal pigmented epithelial cells, pancreatic β-cells, among others. Product Properties Synonyms LN521 ，LAMB2,LN-11 Source Human cell Endotoxin 95% TSE/BSE Animal-Free Concentration 0.1 mg/ml Formulation Clear colorless, buffered solution with a pH of 7.4 with PBS. Bioactivity -Stem Cell Culture Human Laminin 521 Protein could support the rapid expansion of hPSCs. Shipping and Storage This product should be stored at -25~-15℃ for 2 years from date of receipt. 3 months at 2-8°C after thaw. Cautions This product is for research use only. Please operate with lab coats and disposable gloves，foryour safety.
