Mesothelin is derived from a precursor-Megakaryocyte Potentiating Factor (MPF). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression. Specifications Synonyms MSLN, CAK1, Mesothelin, MPF, MPFSMRP, SMR Accession Q13421-2 Source HEK293 Cells-derived Human MSLN/Mesothelin Protein, Glu296-Asp580 with hFc tag at the C-terminal Molecular Weight The protein has a predicted MW of 35.88 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Tris-Bis PAGE result. Purity > 95% by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only. Product Data
