Product Propertie Alternative Names Mesothelin,MPFSMRP,CAK1,MSLN Species Human Expression Sequence Ala488-Ser600 Accession Q13421-1 Tag C-hFc Expression System HEK293 Molecular Weight The protein has a predicted MW of 39.30 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】Immobilized Human MSLN, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-MSLN Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -85 to -65°C. The shelf life is one year after receipt of the product.After reconstitution, store under sterile conditions at -85 to -65°C. The shelf life is three months.
