Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein, hFc-Avi Tag

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Synonyms CD3, CD3e, CD3E, CD3d, T3D, CD3D, CD3E&CD3D, CD3 delta&CD3 epsilon Species Human Expression Sequence Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D) Accession P07766(CD3E)&P04234( CD3D) Tag C-hFc-Avi Expression System HEK293 Molecular Weight The protein has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 48-60 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin Level < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.1ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge the vial briefly. Reconstitute the lyophilized protein with distilled water to a final concentration above 100 µg/mL. For first-time use, it is recommended to aliquot the protein according to your experimental needs to avoid repeated freeze–thaw cycles. Feature High purity, high activity, low endotoxin level, and excellent batch-to-batch consistency. Storage Store at -25°C to -15°C with a 1-year shelf life from the date of receipt.After reconstitution, store under sterile conditions at -85°C to -65°C and use within 3 months. Show more