Mouse Fc gamma RIIB/CD32b Protein, His Tag

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Specifications Source Recombinant Mouse Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag at the C-terminal. It contains Thr40-Arg217.[Uniprot | NP_001070657.1] Mol ecular Weight The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity SPR Data: OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIIB, His Tag with an affinity constant of 0.280 μM as determined in SPR assay Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only. Show more