Specifications Synonyms NKG2-A; NKG2A;CD159a; NKG2A&CD94 Source Human NKG2A&CD94 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-Terminus. It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).[Accession | P26715-1(NKG2A)&Q13241-1(CD94)] Molecular Weight The protein has a predicted MW of 41.1 kDa (NKG2A)&17.9 kDa (CD94). Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human NKG2A&CD94, hFc Tag at 2μg/ml (100μl/Well) on Fc Antibody (2μg/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 40.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only.
