Specifications Synonyms CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94 Source Human NKG2A&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).[Accession | P26715-1(NKG2A)&Q13241-1(CD94)] Molecular Weight The protein has a predicted MW of 17.9 kDa (CD94)&18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on SDS-PAGEresult. Endotoxin 95% as determined by SDS-PAGE and HPLC Activity ELISA Data: Immobilized Human NKG2A&CD94, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only.
