Biotinylated Human Mature TGF beta 3 Protein, Avi Tag

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Specifications Synonyms TGFB3; LAP;TGF-beta-3; ARVD1 Source Biotinylated Human Mature TGF beta 3 Protein is expressed from HEK293 with Avi tag at the C-Terminus. It contains Ala301 -Ser412.[Accession | P10600-1] Molecular Weight The protein has a predicted MW of 14.53 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human TGF-beta RII, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 3, Avi Tag with the EC50 of 6.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCl. Storage The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only. Show more