CD24, also known as Heat-Stable Antigen and Nectadrin, is a heavily and variably glycosylated 30 kDa 60 kDa GPI-linked sialoprotein. Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells. In mouse, CD24 is even more widely expressed, particularly on T cells, monocytes, and dendritic cells. CD24 expression is regulated during lineage development and with the activation of various cell types. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance . CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 associates in cis with Siglec-10 (or Siglec-G in mouse) and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells. Formation of these ternary complexes fills a protective role: the resulting Siglec-10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs. Specifications Synonyms CD 24; CD24 molecule; CD24A; FLJ22950; FLJ43543; MGC75043 Uniprot No. P25063.1 Source Recombinant Human CD24 Protein is expressed from HEK293 Cells with mFc tag at the C-terminal. It contains Ser27-Gly59. Molecular Weight Approximately 29.5 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human CD24, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 0.19μg/ml determined by ELISA. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage Transported with ice packs. Store at -20℃ to -80℃, valid for one year. After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days.。 It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing. Note 1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only. Product Data
