Specification Synonyms Hepassocin; FGL-1;FGL1; HFREP-1 Source Biotinylated Human FGL1 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Asp64-Asn305.[Accession | Q08830] Molecular Weight The protein has a predicted MW of 31.9 kDa same as SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Biotinylated Human FGL1, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.16 μg/ml determined by ELISA. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
