Product Properties Alternative Names FGL1, FGL-1, Hepassocin, HP-041, HFREP-1, LFIRE-1, HFREP1, Lag3 ligand Species Human Expression Sequence Asp64-Asn305 Accession Q08830 Tag N-hFc Expression System HEK293 Molecular Weight The protein has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE,> 90% as determined by HPLC Endotoxin < 1.0 EU per μg by the LAL method. Formulation Supplied as 0.22μm filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Appearance Liquid Biological Activity 【ELISA Data】 Immobilized Human LAG3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGL1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -85 ~ -65℃, valid for 1 year after receipt. Avoid repeated freezing and thawing, and centrifuge before opening the vial. It is recommended to aliquot for one experiment, and freeze at -80℃ after aliquoting.