Human TRAIL R4/TNFRSF10D Protein, His-Avi Tag _ 93834ES

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Specification Source Human TRAIL R4/TNFRSF10D Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala56-His211.[Accession | Q9UBN6] Molecular W eight The protein has a predicted MW of 19.4 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Human TRAIL, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human TRAIL R4, His Tag with the EC50 of 76.1 ng/ml determined by ELISA. SPR Data: Human TRAIL R4, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.13 nM as determined in SPR assay Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Show more