Biotinylated Human TRAIL R4/TNFRSF10D Protein, His-Avi Tag _ 93835ES

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Specification Synonyms TRAILR4; RSF10D;CD264; TRUNDD Source Biotinylated Human TRAIL R4/TNFRSF10D Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It co ntains Ala56-His211.[Accession | Q9UBN6] Molecular Weight The protein has a predicted MW of 19.4 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human TRAIL, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human TRAIL R4, His Tag with the EC50 of 0.14 μg/ml determined by ELISA . Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Caution 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only. Show more