Product Properties Source Recombinant Human Complement Factor D/CFD Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Ile26-Ala253.[Uniprot | P00746-1] Molecular Weight The protein has a predicted MW of 51.2 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE Activity ELISA Data:Immobilized Human Complement Factor D, hFc Tag at 2μg/ml (100 μl/Well) on the plate. Dose response curve for Biotinylated Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 33.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
