Synonyms TGF beta1; TGFB1; DPD1; CEDLAP Source Human LAP (TGF beta 1) Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Leu30-Arg278(C33S). [Accession | P01137] Molecular Weight The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1 EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human LAP (TGF beta 1) , His Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 1.0 μg/mL determined by ELISA. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Specifications Cat.No. Size Price 95230ES25 25 μg $225 95230ES60 100 μg $750 95230ES76 500 μg $1780 Shipping and Storage This product should be stored at -25~-15℃ for one year. After reconstitution, Store at 2–8 °C for 2–7 days under sterile conditions. Store at for 3–6 months under sterile conditions. Aliquot upon first use to avoid repeated freeze-thaw cycles. Documents: Safety Data Sheet 95230_MSDS_HB250605_EN.PDF Manuals 95230_Manual_Ver.EN20250605.pdf Citations & References: