Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, His-Avi Tag

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Product Properties Alternative Names αVβ5, Integrin alpha V beta 5, beta5, alphaV, ITGAV&ITGB5Integrin alpha V β 5, β5 Species Human Expression Sequence Phe31-Val992(ITGAV) acidic tail&Gly24-Asn719(ITGB5) basic tail Accession P06756-1(ITGAV)&P18084(ITGB5) Tag C-His-Avi Expression System HEK293 Molecular Weight The protein has a predicted MW of 114 kDa (ITGAV)&81.4 kDa (ITGB5). Due to glycosylation, the protein migrates to 85-140 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Anti-alpha V Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB5, His Tag with the EC50 of 0.29ug/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months. Show more