Product Properties Alternative Names CD3, CD3G, CD3g, T3G, CD3e, CD3E, CD3 epsilon&CD3 gamma, CD3E&CD3G Species Cynomolgus Expression Sequence Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G) Accession Q95LI5.2(CD3E)&Q95LI7(CD3G) Tag C-His-Flag Expression System HEK293 Molecular Weight The protein has a predicted MW of 11.93 kDa (CD3E) and 11.52 kDa (CD3G). Due to glycosylation, the protein migrates to 13-23 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Appearance Liquid Biological Activity 【ELISA Data】 Immobilized Cynomolgus CD3E&CD3G, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -85 ~ -65℃, valid for 1 year after receipt. Avoid repeated freezing and thawing, and centrifuge before opening the vial. It is recommended to aliquot for one experiment, and freeze at -80℃ after aliquoting.