Product Properties Alternative Names CD221, IGF1R, IGF-1R, IGF-I R, IGF-I receptor, IGFIR, IGFR, JTK13, MGC142170, MGC142172, MGC18216 Species Cynomolgus Expression Sequence Glu31-Asn908 Accession G7P9I7 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 80.59 kDa (alpha subunit) and 19.28 kDa (beta subunit). Due to glycosylation, the protein migrates to 48-58 kDa (beta subunit) and 100-115 kDa (alpha subunit) and 130-150 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Cynomolgus IGF1R, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IGF1R Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
