Product Properties Alternative Names PDCD1, PD1, CD279, SLEB2, PD-1 Species Cynomolgus Expression Sequence Pro21-Gln167 Accession B0LAJ3 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 17.53 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Cynomolgus PD-1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
