Product Properties Alternative Names CD326, EGP, EGP-2, EGP314, EGP40, EpCAM, ACSTD1, DIAR5, gp40, HNPCC8, MOC31, TACST-1, TACSTD1, TROP1, ESA, KSA, MIC18, MK1 Species Cynomolgus,Rhesus macaque Expression Sequence Gln24-Lys265 Accession NP_001035118.1 Tag No Tag Expression System HEK293 Molecular Weight The protein has a predicted MW of 27.38 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Rhesus macaque/Cynomolgus EpCAM, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 0.14μg/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.