Mouse APRIL/TNFSF13 Protein, His-Flag Tag

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Product Properties Alternative Names CD256, TALL2, TALL-2, TNFSF13, APRIL, APRILFLJ57090, TRDL1, TRDL-1, ZTNF2 Species Mouse Expression Sequence Ala96-Leu241 Accession Q9D777 Tag N -His-Flag Expression System HEK293 Molecular Weight The protein has a predicted MW of 18.53 kDa. Due to glycosylation, the protein migrates to 24-30 kDa based on SDS-PAGE result. Purity > 90% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Mouse APRIL, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months. Show more