Product Properties Alternative Names t-PA, tPA, Alteplase, Reteplase, PLAT, Species Mouse Expression Sequence Ser33-Gln559, which consists of two chains: chain A (Ser36-Arg308) and chain B (Ile309-Gln559) Accession P11214 Tag N-hFc Expression System HEK293 Molecular Weight The protein has a predicted MW of 85.00 kDa. The protein is activated by thermolysin and cleaved to a 2-chain protease, it migrates to 35-40 kDa (Chain A) and 30-35 kDa (Chain B) based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.
